吴 毅，副教授，
合成生物学（synthetic biology)：合成基因组学(synthetic genomics)，基因组工程(genome engineering)
1.酵母基因组设计与合成
2.基因组重排与物种进化
3.哺乳动物人工染色体设计与合成
4.Mb级别DNA高效组装与递送技术
代表性论文（*通讯作者，#第一作者）：
H. Xu, M. Han, S. Zhou, B. Z. Li,
Y. Wu
*, Y. J. Yuan (2020).
“
Chromosome drives via CRISPR-Cas9 in yeast
.”
Nature Communications
11(1): 4344.
P. Wang, H. Xu, H. Li, H. Chen, S. Zhou, F. Tian, B. Z. Li, X. Bo,
Y. Wu
*
, Y. J. Yuan (2020). “
SCRaMbLEing of a synthetic yeast chromosome with clustered essential genes reveals synthetic lethal interactions
.”
ACS Synthetic Biology
9 (5), 1181-1189.
Y. Wu
#
, B. Z. Li
#
, M. Zhao, L. A. Mitchell, Z. X. Xie, Q. H. Lin, X. Wang, W. H. Xiao, Y. Wang, X. Zhou, H. Liu, X. Li, M. Z. Ding, D. Liu, L. Zhang, B. L. Liu, X. L. Wu, F. F. Li, X. T. Dong, B. Jia, W. Z. Zhang, G. Z. Jiang, Y. Liu, X. Bai, T. Q. Song, Y. Chen, S. J. Zhou, R. Y. Zhu, F. Gao, Z. Kuang, X. Wang, M. Shen, K. Yang, G. Stracquadanio, S. M. Richardson, Y. Lin, L. Wang, R. Walker, Y. Luo, P. S. Ma, H. Yang, Y. Cai, J. Dai, J. S. Bader, J. D. Boeke, Y. J. Yuan* (2017). "
Bug mapping and fitness testing of chemically synthesized chromosome X
."
Science
355(6329) eaaf4706.
Y
. Wu
#
, R. Y. Zhu
#
, L. A. Mitchell, L. Ma, R. Liu, M. Zhao, B. Jia, H. Xu, Y. X. Li, Z. M. Yang, Y. Ma, X. Li, H. Liu, D. Liu, W. H. Xiao, X. Zhou, B. Z. Li, Y. J. Yuan, J. D. Boeke* (2018). "
In vitro DNA SCRaMbLE
."
Nature Communications
9(1): 1935.
L. Ma, Y. Li, X. Chen, M. Ding,
Y. Wu
*
, Y. J. Yuan (2019). “
SCRaMbLE generates evolved yeasts with increased alkali tolerance
.”
Microbial Cell Factories
18(1): 52
M. J. Shen
#
,
Y.
Wu
#
, K. Yang, Y. Li, H. Xu, H. Zhang, B. Z. Li, X. Li, W. H. Xiao, X. Zhou, L. A. Mitchell, J. S. Bader, Y. Yuan and J. D. Boeke* (2018). "
Heterozygous diploid and interspecies SCRaMbLEing
."
Nature Communications
9(1): 1934.
B. Jia
#
,
Y.
Wu
#
, B. Z. Li
#
, L. A. Mitchell, H. Liu, S. Pan, J. Wang, H. R. Zhang, N. Jia, B. Li, M. Shen, Z. X. Xie, D. Liu, Y. X. Cao, X. Li, X. Zhou, H. Qi, J. D. Boeke and Y. J. Yuan* (2018). "
Precise control of SCRaMbLE in synthetic haploid and diploid yeast
."
Nature Communications
9(1): 1933.
Y. X. Li
#
,
Y. Wu
#
, L. Ma, Z. Guo, W. Xiao, Y. J. Yuan* (2019). “
Loss of heterozygosity by SCRaMbLEing
.”
SCIENCE CHINA Life Sciences
62(3): 381-393
